FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002466640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563964
Sequences flagged as poor quality0
Sequence length289
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCCGT13230123.459121504209488No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTCTG7013912.436786745253244No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCCGG5879310.424956202878198No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCCTG397967.056478782333624No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCCGC391036.933598598492102No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGA329725.846472469873964No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTC294945.22976643899256No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTC201313.5695540850125185No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACCTG175233.1071132199927654No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCCTG123792.1949982623004303No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCCTA107141.899766651772099No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCCTG82751.467292238511678No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCCGG79821.4153385677099957No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCTGG46890.8314360491095176No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAGGT16840.29860061989772396No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGCCC15550.27572681944237576No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGG14520.2574632423346171No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCCAA14260.25285301898702756No Hit
CGACCTCGGGTGGGAACACCTTCCCGTACTCTGCGTTGATACCACTGCTT10620.18830989212077368Clontech SMART CDS Primer II A (96% over 26bp)
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCTTAGGCTGCTGG9890.17536580349100298No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTACTCTAGCACGGTGAGCCGT9060.1606485520352363No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGATG7890.13990254697108326No Hit
CGACCTCGGGTGGGAACACGTTTTTCAAGTCCTCGAGCACCAGGAGCCGC6870.12181628614592421No Hit
CGACCTCGGGTGGGAACACCTTGTTCACCCGTACTCTGCGTTGATACCAC6610.11720606279833465Clontech SMART CDS Primer II A (95% over 21bp)
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGCAGTATTTGAATAA6520.11561021625493825No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCCGTACTCTGCGTT6170.1094041463639523No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCCCGTACTCTGCGTTGATA5880.10426197416856396No Hit
CGACATCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCCGT5680.10071564851657198No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTAACTGTGAGTCTGGTGC5660.10036101595137278No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)