FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002466646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312169
Sequences flagged as poor quality0
Sequence length289
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACCTG5026316.101214406299153No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTC4910915.73154285018692No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCCTG307539.85139459715731No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCCGT223467.15830207355631No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCCGG215846.914203524373017No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCTGG141364.528316392723172No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGA125854.031470133165048No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCCGG109303.501308586054349No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC105803.3891898298677963No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTCTG97363.1188234578065086No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCCTA73522.355134558524389No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCCGC48261.5459574781608678No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCCTG42151.3502301637894858No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTC41391.3258843767318345No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCCAA39081.2518859976487096No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCCTG21520.6893701808956046No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAGGT18760.6009565331599229No Hit
CCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC11700.3747969849664765Illumina Paired End PCR Primer 2 (100% over 48bp)
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGG11380.3645461272579917No Hit
CGACCTCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC9810.314252856625738Illumina Paired End PCR Primer 2 (100% over 45bp)
CGACCTCGGGTGGGAACACGTTTTCCAGGTCCTCTGTGACCGTGAGCCTG6920.22167479794598438No Hit
CAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC6660.21334597605784048Illumina Paired End PCR Primer 2 (100% over 48bp)
CGACCTCGGGTGGGAACACGTTTTTCAGCCCGTACTCTGCGTTGATACCA6320.20245443974257535No Hit
CTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC5950.19060188551713975Illumina Paired End PCR Primer 2 (100% over 48bp)
CGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC5930.18996120691035945Illumina Paired End PCR Primer 2 (100% over 48bp)
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAGGCACTGTCCTC5050.16177134821202616No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGGGCACTGTCAGCCGG4960.1588882944815148No Hit
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC4540.1454340437391285Illumina Paired End PCR Primer 2 (100% over 49bp)
CGACCTCGGGGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC4530.14511370443573834No Hit
CGACCTCGGGTGGGAACACCTGGTTCAGGTCCTCTACAACGGTTAACCTG4070.1303780964797914No Hit
CGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGATG3700.11852554225435581No Hit
GTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC3650.11692384573740505Illumina Paired End PCR Primer 2 (100% over 48bp)
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTAGAGCACTGTCAGCCGG3600.1153221492204543No Hit
CGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCAACTGAGTAATGAATC3560.1140407920068937No Hit
ACAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC3510.11243909548994295Illumina Paired End PCR Primer 2 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)