FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002466656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646222
Sequences flagged as poor quality0
Sequence length289
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAACAAATA21920833.92146971164708No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATAACGGTGA453897.023747257134545No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAA352035.447508750862707No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGA157742.440956822887491No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTTACAGTTA146382.265165840841073No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCACGGACA131542.0355233959846615No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACTGTCA103821.6065686404981567No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA93681.449656619551795No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGACAGTCA83281.288721213452962No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCA82131.2709254714324179No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATA78851.2201689202781707No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGGACAGTCA78621.2166097718740618No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTGACTCTCA73631.1393917260631796No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACTGATA63540.9832534330307542No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATCACAGAAA54360.8411969880319767No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTGACCACAA53570.8289720869917768No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA51900.8031295746662911No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAGACTTGTA50710.7847148503145978No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGA44730.6921769918077689No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAAACAGTCA40730.6302787586928331No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACTTGGA37910.5866405043468035No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGACTAGAA37070.573641875392667No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGATGCTGA35850.5547629142926116No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTA32840.5081844938736224No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTCACGGTTA32290.49967348682031876No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA31450.4866748578661822No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACATTGA29560.45742794271937504No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA29420.4552615045603523No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATAGTCA28810.4458220240103246No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGA27550.4263240805791198No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA26850.41549188978400614No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA26340.4075998650618518No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGGACAGCAA23300.36055720789450063No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAAGTCCTGGGGCT22410.3467848510264274No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACCACCA22190.343380448205106No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCCACCTTTA21820.33765486164197445No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTGACCAACA19970.30902692882631666No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGACCAGAA19820.30670574508450654No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA19560.30268235993203574No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAAACAATCA18700.2893742398123246No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTTCA15080.23335633884330773No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTA12450.19265825057023747No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTTATAATTA11400.17640996437756684No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGTTGTTGCCAGCCC11120.17207708805952135No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAGAGATTAACA8660.13400967469383587No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA8630.13354543794547385No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACTGCCA7930.12271324715036008No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTCACTGTGA7600.1176066429183779No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACTAGCA6930.10723868887162616No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGA6610.10228683022243129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)