FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002466662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002466662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327925
Sequences flagged as poor quality0
Sequence length289
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGGACAGTCA216406.599069909278037No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACCGTCA186315.681482046199588No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTTACTTGTA183805.604940153998628No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGGATGATTA141134.303727986582298No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACTAGCA139074.240908744377525No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTAACAGAGA132984.0551955477624455No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAGACTTGTA132254.032934359990851No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGAATATGAA106053.233971182435008No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTTACAGTTA100833.074788442479225No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTGACCATTA98062.9903179080582447No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGGGTGACCACAA96892.954639018068156No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATGACTTGGA90902.771975299230007No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGAACAATAA81752.4929480826408477No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTTATAATTA78992.4087824959975603No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTCACAGATA75662.307234886025768No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATCACAGAAA73592.2441106960433026No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATGATGGTGA67602.061446977205154No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGACTTGACTCTCA64091.954410307234886No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGCACGGACA63791.945261873904094No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAACAAATA56701.7290538995197071No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTCACAGTGA55911.704963025081955No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAGACTAGAA53751.6390943051002516No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCCACTTTTA50301.5338873217961424No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTCACCACCA44321.3515285507356865No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACATTGA44211.3481741251810628No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATGACCGAGA43131.3152397651902112No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAAACCTTCA41451.2640085385377755No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATGACTGATA37491.1432492185713197No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGTACAGCCA36581.1154989708012504No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTTACTGTCA35071.0694518563695967No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTCACTGTGA31960.9746130975070519No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTGATAGTCA30440.9282610352977052No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAAACAGTCA28990.8840436075322101No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTGTGACCAACA27970.852938934207517No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTGACCTGAA27880.8501944042082793No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGGATGCTGA24660.7520012197911108No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTTACTGTCA24230.7388884653503087No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTGGTAAAACAATCA24220.7385835175726158No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTCACTGATA23160.7062590531371503No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGACCAGAA22820.6958908286955859No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAGACAGTCA22780.6946710375848135No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTCACGGTTA21310.6498437142639323No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCCACCTTTA20660.6300221087138828No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTCTAACACTCA20490.6248379964931006No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGCACTTGGA19680.6001372264999619No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATAACGGTGA18510.5644583365098728No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTTACTGCCA15440.4708393687581002No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGGACAGCAA10630.32415948768773345No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTGACCAGCA9200.28055195547762446No Hit
CAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTTACTGTCA4110.1253335366318518No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGAAGAGATAAGGT3750.11435541663490127No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCAAGTCCTGGGGCT3730.11374552107951515No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATACTTGGCTGGACAGCAA3590.10947625219181216No Hit
CAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGTACTTGGCTGGACA3500.10673172219257453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)