FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002481334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54039127
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC3377170.6249490299871054No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC2039680.3774450316342083No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG1919160.3551426728266724No Hit
CTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG1663540.30783991014510653No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC1338290.24765203923446064No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT1309730.24236697976264496No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA1154400.21362299209607885No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG1089510.20161502609026233No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA1026550.18996420871121772No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA927710.17167375779405172No Hit
CTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT849290.15716205037879313No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC785060.1452762181002665No Hit
CACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGT783150.14492277049553373No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA767760.1420748340364566No Hit
GGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCT758340.1403316526560468No Hit
CCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGA753420.1394212012344315No Hit
CCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG745920.138033317969774No Hit
CTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTG740500.13703034099718153No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT737960.13656031119821754No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC728680.13484303697208136No Hit
CTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTG717160.13271124827756747No Hit
TGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA691540.12797023904549754No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT643490.11907853359659196No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA640460.11851782875767033No Hit
GAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA638820.11821434495046523No Hit
GGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAG635680.11763328449032864No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTC630440.11666361671608795No Hit
GTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTG627360.11609365932206861No Hit
CTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGT599950.11102140861750041No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTA588610.10892292912133832No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT576450.10667270772157365No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC558870.10341950934921654No Hit
AGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCT551330.1020242240404809No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC545040.10086025260918816No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC542970.10047719682814268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)