FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002481455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38142099
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT492800.1292010699253861TruSeq Adapter, Index 27 (97% over 39bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG478670.1254965019098713No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT442720.11607122093621539No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT389970.10224135803328495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)