FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002481595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38157998
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26378586.912988464436736No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14577433.820281661527421TruSeq Adapter, Index 15 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9081622.380004317836591No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8120332.1280807237318897No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2138220.560359586999297TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA779370.2042481369174557TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT484950.12709000089574932TruSeq Adapter, Index 15 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC482970.1265711057482628TruSeq Adapter, Index 15 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)