FastQCFastQC Report
Wed 8 May 2024
EGAF00002481718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67893053
Total Bases543.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC2737240640.316946713237364No Hit
AGCCAATT1984584129.231033401900486No Hit
CTAGGTGA1916184328.22356950128609RNA PCR Primer, Index 38 (100% over 8bp)
TCGTTCAG3132040.46131965813939757No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)