FastQCFastQC Report
Thu 9 May 2024
EGAF00002481749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31450284
Total Bases251.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA1028449032.70078578622692No Hit
CCAAGATG730254123.219316556887055No Hit
GTTTATCT680271321.630052688872382No Hit
TACGTGAC660870921.013193394374436RNA PCR Primer, Index 17 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)