FastQCFastQC Report
Fri 10 May 2024
EGAF00002481750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32496448
Total Bases259.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA1068031232.86609047241102No Hit
CCAAGATG757380023.306547226330707No Hit
GTTTATCT705895321.722229457200985No Hit
TACGTGAC688233821.178739288675487RNA PCR Primer, Index 17 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)