FastQCFastQC Report
Wed 8 May 2024
EGAF00002481852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67797099
Total Bases542.3 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTCTC2019544629.788068070582195No Hit
TTCGCTGA1822544826.8823419715938No Hit
CATTAGCG1428620221.071996015640728No Hit
ACAAGAAT1347971619.882437742653266No Hit
CATTAGCT1639230.24178468167199899No Hit
GGGATCTC1175850.17343662447857836No Hit
GGGGTCTC779200.11493117131752201No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)