FastQCFastQC Report
Wed 8 May 2024
EGAF00002481885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66608645
Total Bases532.8 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACC2196335432.97372886057058No Hit
AGCGCCTA1970768029.5872705412338No Hit
TTGTTGAT1309741719.663238908402956No Hit
CAAAGTGG1114592916.733457046003565ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)