FastQCFastQC Report
Wed 8 May 2024
EGAF00002481886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67521827
Total Bases540.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACC2227235832.98541966288916No Hit
AGCGCCTA1996044029.56146313991178No Hit
TTGTTGAT1327211419.65603507736839No Hit
CAAAGTGG1128378116.711308774272354ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GCTCAACA703830.10423740459510966No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)