FastQCFastQC Report
Mon 13 May 2024
EGAF00002481896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58489185
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAG810780.13862049881529379No Hit
GTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGG697770.11929897809312953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG637970.10907486572090208Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT616610.10542290852573856No Hit
GCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCAT596410.1019692785939828No Hit
GGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCAT593540.1014785895888274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)