FastQCFastQC Report
Wed 8 May 2024
EGAF00002481995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64967666
Total Bases519.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA2023489531.146101200557215No Hit
CCAAGATG1654340925.464065462964296No Hit
GTTTATCT1466069922.566146981484607No Hit
TACGTGAC1280103019.703693834406796RNA PCR Primer, Index 17 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)