FastQCFastQC Report
Wed 8 May 2024
EGAF00002481996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002481996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65398075
Total Bases523.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA2037744631.15909145643813No Hit
CCAAGATG1664879025.45761476924206No Hit
GTTTATCT1478247822.603842697204772No Hit
TACGTGAC1291818619.75315940109858RNA PCR Primer, Index 17 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)