FastQCFastQC Report
Wed 22 Mar 2023
EGAF00002482054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58602176
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1723790.29415119329357325Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1185030.20221604057842493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1173490.2002468304248634Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGACCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCT757780.12930919152217146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACACACACACACACAC735600.12552434912997087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACCAACCTCCACA653430.11150268549754876No Hit
GTGACCACCTGACCAACCTCCACAGGCTGGGTGGCCCGGAGGCTGGGCTG627740.1071188892371505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)