FastQCFastQC Report
Sun 14 May 2023
EGAF00002482419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54888960
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33796606.157267326617228No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15099812.7509739663495174Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAT1224370.22306307133529218Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)