FastQCFastQC Report
Sat 4 May 2024
EGAF00002482420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54888960
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16194522.9504148010820392Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5882811.071765615526328No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT835340.15218725222704163No Hit
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT746080.135925329975281Illumina Paired End Adapter 2 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)