FastQCFastQC Report
Sun 12 May 2024
EGAF00002482421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55043443
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41804947.594899178091022No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATCTCGTA15365352.791495074172595Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1362410.24751540342416445Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)