FastQCFastQC Report
Sun 14 May 2023
EGAF00002482421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55043443
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500176.267807411683894No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15253202.7711202585928354Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCAGAT1231330.22370148611524898Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)