FastQCFastQC Report
Fri 3 May 2024
EGAF00002482422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55043443
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16232172.948974321973282Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5538841.0062669953258556No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT885780.16092379977030144No Hit
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT683490.12417282836031897Illumina Paired End Adapter 2 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)