FastQCFastQC Report
Fri 10 May 2024
EGAF00002482423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51610140
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTA50569649.798392331429444Illumina Paired End PCR Primer 2 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25431954.927704129459832No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG5227661.0129133538486816Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA1945840.3770266850661517Illumina Paired End Adapter 2 (100% over 32bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1703670.3301037354287355Illumina Paired End PCR Primer 2 (100% over 31bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTAT531490.102981700882811Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)