FastQCFastQC Report
Sat 13 May 2023
EGAF00002482423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51610140
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50155489.71814453516305Illumina Paired End PCR Primer 2 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20741194.018820720114303No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGAT4734760.9174088657771515Illumina Paired End PCR Primer 2 (97% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGA1812530.35119648968206635Illumina Paired End PCR Primer 2 (97% over 38bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTATGCCG1569070.3040235891629048Illumina Paired End PCR Primer 2 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)