FastQCFastQC Report
Mon 6 May 2024
EGAF00002482424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51610140
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG536900010.402994450315383Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3348590.6488240489175189No Hit
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT2894790.5608955914477272Illumina Paired End Adapter 2 (100% over 30bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1768250.3426167803458778Illumina Single End PCR Primer 1 (100% over 31bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT781790.15147992235634314Illumina Paired End Adapter 2 (96% over 30bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556880.10790127676460479No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)