FastQCFastQC Report
Fri 10 May 2024
EGAF00002482425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002482425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51758486
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTA51004079.854243031761015Illumina Paired End PCR Primer 2 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26096165.0419094561614495No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG5293911.0228100566929257Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA1965840.37981018223755614Illumina Paired End Adapter 2 (100% over 32bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1729660.3341790175238124Illumina Paired End PCR Primer 2 (100% over 31bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTAT577950.11166284887081125Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)