FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257417
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA155486.040005127866458No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA89243.46674850534347No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA88123.4232393353974295No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA80353.1213944688967707No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA72362.8110031582995685No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA58032.2543188678292423No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA57512.234118181782866No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA56642.200320880128352No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA52402.0356075939040545No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG41561.6145009847834446No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG34281.3316913801341792No Hit
GACTACTGGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC28111.0920024706992932No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG23960.9307854570599455No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG23120.8981535796004149No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23040.8950457817471262No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG20710.8045311692700948No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG20700.8041426945384338No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG19920.7738416654688696No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG19190.7454830100576109No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG18970.736936565961067No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG18050.7011968906482478No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA17910.6957582444049927No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG17670.6864348508451268No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA17250.6701189121153615No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG15370.5970856625630786No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG14980.5819351480282965No Hit
GACTACTCGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC14590.5667846334935145No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG14430.5605690377869371No Hit
GACTACAGGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC14140.5493032705687658No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG13610.5287141097907286No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13530.52560631193744No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG13040.5065710500860471No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG13030.506182575354386No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12920.5019093533061142No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG12790.49685918179452015No Hit
GACTACTAGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC12440.4832625661863824No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG12330.4789893441381105No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG11980.46539272852997277No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA11970.46500425379831173No Hit
GACTACCGGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC11490.4463574666785799No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA11330.44014187097200264No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG11000.427322204827187No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA10670.41450253868237136No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10580.41100626609742164No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG10310.4005174483425726No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9970.3873093074660959No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA9470.3678855708830419No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9190.3570082783965317No Hit
GACTACACGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC9020.35040420795829336No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8740.3395269154717831No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8550.3321458955702226No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8400.32631877459530645No Hit
GACTACCAGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC8210.31893775469374597No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG8040.3123336842555076No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG7930.30806046220723576No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7830.30417571489062495No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG7710.29951401811069195No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7710.29951401811069195No Hit
GACTACTGGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG7630.2964062202574033No Hit
GACTACCCGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC7600.2952407960624201No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7560.29368689713577584No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG7520.29213299820913147No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7510.2917445234774704No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG7440.28902520035584284No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG7390.2870828266975375No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG7330.284751978307571No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6970.2707668879677721No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6930.26921298904112784No Hit
GACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6910.2684360395778057No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6790.2637743427978727No Hit
GACTACAAGGGTATCACTTAGTTTGGGAGGTAGAAATTAGCCTCTTCAAC6780.2633858680662116No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6600.25639332289631217No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6410.2490123029947517No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG6390.24823535353142956No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA6140.23852348523990258No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG6130.23813501050824148No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG5980.2323078895333253No Hit
GACTACTGGGGTATCTAATCCCGTCTAAACTATTGCAGCCACCCGTAGG5650.2194882233885097No Hit
GACTACTGGGGTATCTAATCCTACAGTTAATGTTGGGAGGATGATGCTCT5230.20317228465874435No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG5170.20084143626877793No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4980.1934604163672174No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG4930.19151804270891198No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4920.19112956797725092No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG4890.1899641437822677No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4880.1895756690506066No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG4870.1891871943189455No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4850.18841024485562338No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4800.18646787119731797No Hit
GACTACTCGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG4730.1837485480756904No Hit
GACTACTGGGGTATCTAATCCCGTCTAAACTATTGCAGCCGCCCGTAGG4690.1821946491490461No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG4680.18180617441738503No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4570.17753295236911315No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG4420.17170583139419696No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4400.1709288819308748No Hit
GACTACTGGGGTATCTAATCCCGTCTAAACTATTGCAGCCTCCCGTAGG4330.16820955880924726No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG4310.1674326093459251No Hit
GACTACAGGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG4280.16626718515094185No Hit
GACTACTAGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG4250.16510176095595863No Hit
GACTACTGGGGTATCTAATCCATCTGCAGCCCCCCGTAGG4160.1616054883710089No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCGCCTCAG3980.1546129432011095No Hit
GACTACCGGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG3690.1433471759829382No Hit
GACTACTGGGGTATCTAATCCGTTCTCCAGCCTGCAGCCCCCCGTAGG3680.1429587012512771No Hit
GACTACTGGGGTATCTAATCCGACTACTGCAGCCCCCCGTAGG3620.14062785286131063No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3430.13324683295975012No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3360.13052750983812259No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3330.12936208564313934No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG3330.12936208564313934No Hit
GACTACAGGGGTATCTAATCCTACAGTTAATGTTGGGAGGATGATGCTCT3290.12780818671649502No Hit
GACTACTCGGGTATCTAATCCCGTCTAAACTATTGCAGCCACCCGTAGG3280.12741971198483396No Hit
GACTACCAGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG3230.12547733832652855No Hit
GACTACAGGGGTATCTAATCCCGTCTAAACTATTGCAGCCACCCGTAGG3220.12508886359486748No Hit
GACTACTCGGGTATCTAATCCTACAGTTAATGTTGGGAGGATGATGCTCT3220.12508886359486748No Hit
GACTACCCGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG3120.12120411627825668No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3040.11809631842496805No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2980.11576547003500158No Hit
GACTACTAGGGTATCTAATCCTACAGTTAATGTTGGGAGGATGATGCTCT2960.11498852057167941No Hit
GACTACTAGGGTATCTAATCCCGTCTAAACTATTGCAGCCACCCGTAGG2930.11382309637669617No Hit
GACTACACGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG2870.11149224798672971No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2850.11071529852340754No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG2840.11032682379174648No Hit
GACTACCGGGGTATCTAATCCCGTCTAAACTATTGCAGCCACCCGTAGG2740.10644207647513568No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2690.10449970281683027No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG2680.1041112280851692No Hit
GACTACAAGGGTATCTAATCCCGTCTAAACTATTGCAGCCCCCCGTAGG2650.10294580389018596No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2630.1021688544268638No Hit
GACTACAGGGGTATCTAATCCATCTGCAGCCCCCCGTAGG2590.10061495550021948No Hit
GACTACAGGGGTATCTAATCCCGTCTAAACTATTGCAGCCTCCCGTAGG2580.1002264807685584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)