FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213374
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT122415.736875158172973No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC121555.696570341278694No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT109405.127147637481605No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC97484.568504128900428No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT79583.729601544705541No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT79003.70241922633498No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC73363.438094613214356No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC70223.2909351654840795No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC41241.9327565682791716No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC37601.7621640874708258No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT32641.5297083993363765No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC31291.466439210025589No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26701.2513239663689109No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25831.21055048881307No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT23891.1196303204701603No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC22951.0755762182833897No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC22501.054486488513127No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT20410.9565364102467966No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19520.9148256113678329No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT18350.8599923139651504No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC18340.859523653303589No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT17120.8023470525930995No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT17100.8014097312699766No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16980.7957858033312399No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT14170.6640921574324894No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14160.663623496770928No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT13810.6472203736162794No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT12690.5947303795214037No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT12440.583013862982369No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10580.4958429799319505No Hit
CCTACGGGGTGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT10510.49256235530102077No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10360.4855324453775999No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC9870.46256807296109176No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9480.4442903071601976No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT9230.43257379062116286No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC6870.32196987449267483No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6390.29947416273772814No Hit
CCTACGGGGGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC6220.2915069314911845No Hit
CCTACGGGGGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT6080.28494568222932504No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5760.26994854105936056No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5610.2629186311359397No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT5490.25729470319720305No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC5150.24136024070411577No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5070.23761095541162466No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5010.23479899144225633No Hit
CCTACGGGTGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC4950.23198702747288796No Hit
CCTACGGGTGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT4910.2301123848266424No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4810.22542577821102855No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4660.21839586828760765No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT4570.21417792233355518No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4400.2062106910870115No Hit
CCTACGGGAGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT4390.20574203042545014No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4020.18840158594767872No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3960.1855896219783104No Hit
CCTACGGGCGGCTGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT3950.185120961316749No Hit
CCTACGGGGAGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3920.18371497933206482No Hit
CCTACGGGCGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC3910.18324631867050342No Hit
CCTACGGGAGGCTGCAGTGGGGAATTGTTCGCAATGGGCGCAAGCCTGAC3820.17902837271645095No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3650.1710611414699073No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3510.16449989220804784No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3470.1626252495618023No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3470.1626252495618023No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC3410.15981328559243393No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT3400.15934462493087254No Hit
CCTACGGGGTGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3260.1527833756690131No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3100.14528480508403085No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3090.14481614442246946No Hit
CCTACGGGGTGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3030.14200418045310112No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3030.14200418045310112No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2910.13638025251436445No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2890.13544293119124168No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2870.13450560986811888No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2860.1340369492065575No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2850.1335682885449961No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2790.13075632457562775No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC2650.12419507531376832No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2490.11669650472878608No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC2440.11435320142097911No Hit
CCTACGGGGGGCAGCAGCTGCAGGAGGGTTTGGAGAAGAGGCTGCTGTGT2380.11154123745161079No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC2270.1063859701744355No Hit
CCTACGGGGTGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2230.10451132752818994No Hit
CCTACGGTGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2180.102168024220383No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT2160.10123070289726022No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG2140.10029338157413743No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2140.10029338157413743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)