FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279808
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT203757.281778934126258No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT194776.960844579139981No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT141755.065973810612992No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC137874.927307296431839No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT132004.717520585544373No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC119394.266854414455627No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC83022.967034537968893No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC80442.8748284537968893No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT53371.9073793458371453No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC44351.5850154391582798No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT40591.4506375800548947No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT37491.3398473238792314No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT37091.3255518069533394No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT28991.0360675892040256No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28591.0217720722781336No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC27020.9656621683440072No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT26560.9492223238792316No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT26100.9327824794144556No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT25870.9245625571820676No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC25820.9227756175663312No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC23430.8373599039341262No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19340.6911882433668801No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC19100.6826109332113449No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC18870.674391010978957No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC17440.6232845379688929No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC17230.6157793915827997No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC16700.5968378316559927No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC15190.5428722552607502No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT13780.49248055809698077No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT12810.45781392955169264No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT12750.45566960201280876No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT12020.42958028362305584No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11360.40599268069533395No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10970.3920545516925892No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC10820.38669373284537967No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT9500.33951852698993595No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT8680.3102127172918573No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8680.3102127172918573No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT8150.2912711573650503No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT8040.28733989021043005No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTGGGCAATGCACGAAAGTGTGAC7900.2823364592863678No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT7460.2666113906678866No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7260.2594636322049405No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTGGGCAATGCACGAAAGTGTGAC7130.2548175892040256No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG6900.24659766697163768No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6210.22193790027447394No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5980.21371797804208603No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5970.21336059011893868No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5720.20442589204025616No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5460.19513380603842634No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTGGGCAATGCACGAAAGTGTGAC5350.19120253888380603No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG5330.19048776303751144No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTGGGCAATGCACGAAAGTGTGAC4950.176907021957914No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4940.1765496340347667No Hit
CCTACGGGGGGCTGCTTGGATTAGATACCCCAGTAGTC4700.16797232387923147No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4560.16296889295516925No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC4360.15582113449222323No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4280.15296203110704482No Hit
CCTACGGTGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT4110.1468864364135407No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3860.1379517383348582No Hit
CCTACGGGTGGCTGCTTGGATTAGATACCCCAGTAGTC3820.13652218664226898No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3700.13223353156450138No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3690.13187614364135405No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT3660.13080397987191217No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3540.12651532479414457No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3320.11865279048490394No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3210.11472152333028363No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3160.11293458371454711No Hit
CCTACGGGGGGCTGCTTGGATTAGATACCCCTGTAGTC3030.10828854071363221No Hit
CCTACGGGGGGCTGCAGAAGGATTAGATACCCCAGTAGTC3000.10721637694419031No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2960.10578682525160109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)