FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330506
Sequences flagged as poor quality0
Sequence length35-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG159774.834102860462442No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA153384.640762951353379No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG118543.586621725475483No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG91372.7645489038020488No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG90212.7294512051218436No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA84872.567880764645725No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA84832.566670499173994No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG82902.508275190162962No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA76302.3085813873273104No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA69862.113728646378583No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG68752.0801437795380417No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG68372.0686462575565954No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG67792.051097408216492No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG62221.8825679412779195No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG59291.793915995473607No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG54561.65080210344139No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA54501.648986705233793No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA54141.638094315988212No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG53171.6087453782987298No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA52611.5918016616944926No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCACCTCAG51841.5685040513636666No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG51831.5682014849957338No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA48431.4653289198985797No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG42951.299522550271402No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG40261.2181321972974772No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG40191.2160142327219476No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG39591.1978602506459792No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG36661.109208304841667No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG36401.101341579275414No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24440.7394722032277781No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21360.6462817619044737No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21000.6353893726588927No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19870.6011993730824856No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG19750.5975685766672919No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG19190.5806248600630549No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG18850.5703376035533394No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG15700.4750291976545055No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15550.4704907021355134No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13150.39787477383164No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12750.38577211911432774No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12740.38546955274639494No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG12440.37639256170841073No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12350.3736694643970155No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12290.37185406618941863No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG12120.366710437934561No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG11760.3558180486889799No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG11030.33373070382988507No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9190.2780584921302488No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA8590.2599045100542804No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG7740.2341863687799919No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6950.21028362571330023No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6640.20090406830738325No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6570.1987861037318536No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6170.18668344901454134No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG5900.17851415708035556No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCACCCGTAGG5680.17185769698583384No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5580.16883203330650579No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5400.16338583868371528No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG5340.16157044047611843No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA5190.15703194495712633No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG4890.14795495391914218No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4860.14704725481534373No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4750.14371902476808288No Hit
GACTACTCGGGTATCTAATCCTCTGCAGCCCCCCGTAGG4580.13857539651322517No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCGCCCGTAGG4510.13645743193769552No Hit
GACTACAGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG4470.13524716646596432No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4430.1340369009942331No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4390.13282663552250187No Hit
GACTACTAGGGTATCTAATCCTCTGCAGCCCCCCGTAGG4320.13070867094697222No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4230.12798557363557697No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4220.12768300726764414No Hit
GACTACTGGGGTATCTAATCCTCTGCAGCCTCCCGTAGG4100.1240522108524505No Hit
GACTACCGGGGTATCTAATCCTCTGCAGCCCCCCGTAGG3910.11830344986172717No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3820.11558035255033192No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3660.11073929066340701No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3640.11013415792754142No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3610.10922645882374298No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3520.10650336151234772No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3370.10196486599335564No Hit
GACTACAGGGGTATCTAATCCTCTGCAGCCACCCGTAGG3360.10166229962542284No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3330.10075460052162441No Hit
GACTACTCGGGTATCTAATCCTCTGCAGCCACCCGTAGG3320.10045203415369162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)