FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240315
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA85803.570313962923663No Hit
CCTACGGGTGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA70942.9519588872937605No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC67802.8212970476249923No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT59992.4963069304870693No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT57922.410169985227722No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC57762.4035120570917337No Hit
CCTACGGGCGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA53122.2104321411480767No Hit
CCTACGGGAGGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA51822.1563364750431724No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC45121.8775357343486672No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT41181.7135842539999584No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT40671.6923621080664961No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC40371.6798784928115182No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC35161.463079707883403No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC28911.2030043900713647No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT26221.0910679732850634No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT26001.0819133220980797No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT24521.0203274868401888No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT24421.0161662817551964No Hit
CCTACGGGGGGCAGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA23920.9953602563302333No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC22940.9545804464973057No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC22370.9308615775128478No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT22230.925035890393858No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT22210.9242036493768596No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC21600.8988202983584046No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC21200.8821754780184342No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT19420.8081060275055656No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19340.8047770634375715No Hit
CCTACGGGGGGCTGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC18450.7677423381811372No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC17940.7465201922476749No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT17860.7431912281796809No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT17830.7419428666541831No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17630.7336204564841978No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17610.7327882154671993No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT17010.7078209849572437No Hit
CCTACGGGTGGCTGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC16690.6945051286852673No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC15780.6566381624118345No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT15110.6287580883423839No Hit
CCTACGGGTGGCAGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA15050.6262613652913884No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14300.5950523271539437No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT14130.5879782785094563No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13580.565091650541997No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC13540.5634271685079999No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC12860.5351309739300502No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12530.5213989971495745No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC12380.5151571895220856No Hit
CCTACGGGCGGCTGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC12120.5043380563011047No Hit
CCTACGGGAGGCTGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC11730.4881093564696336No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11660.4851965129101388No Hit
CCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA10940.455235836298192No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC10800.4494101491792023No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10570.4398393774837193No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC9640.40114017019328796No Hit
CCTACGGGCGGCAGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA9560.3978112061252939No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC9440.3928177600233027No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC9030.37575681917483306No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT8650.3599442398518611No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7860.32707071968041945No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7790.3241578761209246No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7580.31541934544244016No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7350.3058485737469571No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC7330.3050163327299586No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT7330.3050163327299586No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6760.2812974637455007No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT6670.2775523791690074No Hit
CCTACGGGGTGCTGCAGTAAGGAATATTGGTCAATGGACGCAAGTCTGAA6140.25549799221854647No Hit
CCTACGGGGGGCAGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC5970.24842394357405903No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCAGTAGTC5930.24675946154006198No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5670.23594032831908118No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5510.229282400183093No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC5380.22387283357260263No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5250.2184632669621122No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5210.21679878492811516No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT5040.20972473628362776No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5020.2088924952666292No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT4760.1980733620456484No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4610.1918315544181595No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT4520.18808646984166613No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCCAGTAGTC4510.1876703493331669No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4390.18267690323117575No Hit
CCTACGGGTGGCAGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC4220.1756028545866883No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3960.16478372136570751No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3820.15895803424671784No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC3790.15770967272122008No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3650.1518839856022304No Hit
CCTACGGGAGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC3640.15146786509373114No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3600.14980338305973412No Hit
CCTACGGGAGGCAGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC3580.1489711420427356No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT3540.14730666000873852No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3470.1443938164492437No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT3430.14272933441524666No Hit
CCTACGGGCGGCTGCAGGATTAGATACCCCAGTAGTC3370.14023261136425108No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT3370.14023261136425108No Hit
CCTACGGGTGGCAGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC3360.13981649085575182No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCGAGTAGTC3250.13523916526225996No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCTGTAGTC3120.12982959865176955No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3060.127332875600774No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT3050.1269167550922747No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCTAGTAGTC2930.1219233089902836No Hit
CCTACGGGAGGCTGCAGGATTAGATACCCCAGTAGTC2930.1219233089902836No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2730.11360089882029836No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2730.11360089882029836No Hit
CCTACGGGGGGCTGCAGGATTAGATACCCCGGTAGTC2700.11235253729480058No Hit
CCTACGGGCGGCAGCAGTCGAGAATCTTCCGCAATGGACTAAAGTCTGAC2660.11068805526080353No Hit
CCTACGGGTGGCTGCAGGATTAGATACCCGAGTAGTC2640.109855814243805No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2620.10902357322680649No Hit
CCTACGGGCGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2550.10611072966731165No Hit
CCTACGGGGTGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2450.10194952458231904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)