FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240315
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG80203.337286478164076No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA73443.0559890144185755No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG46621.9399538106235565No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG43961.829265755362753No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG42431.7655993175623659No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA40311.6773817697605227No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA40191.6723883236585315No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA37651.566693714499719No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG35931.4951209870378461No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG32531.3536400141480973No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA32061.334082350248632No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG29551.2296361026153173No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA27221.1326800241349895No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG26541.1043838295570396No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG26501.1027193475230428No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG26331.0956452988785552No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG25431.0581944531136218No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA25151.0465430788756425No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCCTCAG24771.0307304995526705No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24641.02532093294218No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA24521.0203274868401888No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG22470.9350227825978403No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG22240.9254520109023574No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG21090.8775981524249422No Hit
GACTACTGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG20430.850134198863991No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG20220.8413956681855066No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG18150.7552587229261595No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG16850.7011630568212555No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG16840.7007469363127562No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA16510.6870149595322805No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG16320.6791086698707945No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG16110.6703701391923101No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG15770.6562220419033352No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG15500.6449867881738551No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG14140.5883943990179556No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG14080.58589767596696No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG13240.550943553253022No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG13060.5434533841000354No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG12830.5338826124045524No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12540.5218151176580738No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG12460.5184861535900797No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12450.5180700330815804No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG12380.5151571895220856No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG12310.5122443459625907No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG12200.5076670203690988No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11970.49809624867361585No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG11950.4972640076566174No Hit
GACTACTCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG11080.4610615234171816No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG11060.4602292824001831No Hit
GACTACAGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG11020.458564800366186No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG10960.45606807731519045No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG10350.43068472629673554No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG10200.4244429186692466No Hit
GACTACTAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG9960.41445602646526436No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG9820.4086303393462747No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG9630.4007240496847887No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG9570.3982273266337931No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9300.3869920729043131No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG9230.38407922934481825No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA9190.38241474731082126No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA9000.3745084576493352No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8970.3732600961238374No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8860.3686827705303456No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8840.36785052951334707No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG8760.36452156544535297No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8690.36160872188585813No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8630.3591119988348626No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG8630.3591119988348626No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG8400.3495412271393796No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG8390.3491251066308803No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG8360.3478767451053825No Hit
GACTACACGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG8180.34038657595239585No Hit
GACTACCGGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG8130.3383059734098995No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8100.3370576118844017No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG7840.3262384786634209No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG7790.3241578761209246No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG7780.32374175561242535No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG7550.31417098391694237No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCTCCTCAG7520.3129226223914446No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7500.312090381374446No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG7410.3083452967979527No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACACTTTCGCACATCAG7310.30418409171296007No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7130.2966939225599734No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA7120.2962778020514741No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6930.28837151238998815No Hit
GACTACTGGGGTATCTAATCCTGCAGCCCCCCGTAGG6610.2750556561180118No Hit
GACTACAAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG6490.2700622100160206No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG6190.2575785947610428No Hit
GACTACCCGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG6180.25716247425254357No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA6010.2500884256080561No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6000.24967230509955682No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5830.2425982564550694No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5820.24218213594657012No Hit
GACTACCAGGGTATCTAATCCCATTCGCTACCCACGCTTTCGTGCTTGAG5820.24218213594657012No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5800.2413498949295716No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5570.23177912323408856No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA5440.22636955662359817No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5410.22512119509810039No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5410.22512119509810039No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5340.2222083515386056No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5140.21388594136862038No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5130.2134698208601211No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4960.20639577221563365No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4770.1984894825541477No Hit
GACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4750.19765724153714917No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4740.1972411210286499No Hit
GACTACTGGGGTATCTAATCCTGCAGCCACCCGTAGG4690.1951605184861536No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4540.18891871085866466No Hit
GACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4540.18891871085866466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4430.1843413852651728No Hit
GACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA4420.18392526475667353No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4410.18350914424817427No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4380.18226078272267648No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4140.1722738905186942No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG4080.16977716746769864No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4070.1693610469591994No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4060.1689449264507001No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3850.16020639577221563No Hit
GACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3820.15895803424671784No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3780.15729355221272082No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3720.15479682916172524No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3680.1531323471277282No Hit
GACTACTCGGGTATCTAATCCTGCAGCCCCCCGTAGG3620.15063562407673262No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3600.14980338305973412No Hit
GACTACTGGGGTATCTAATCCTGCAGCCGCCCGTAGG3580.1489711420427356No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3520.14647441899174No Hit
GACTACAGGGGTATCTAATCCTGCAGCCCCCCGTAGG3460.14397769594074444No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3420.1423132139067474No Hit
GACTACTGGGGTATCTAATCCTGCAGCCTCCCGTAGG3360.13981649085575182No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3310.1377358883132555No Hit
GACTACTAGGGTATCTAATCCTGCAGCCCCCCGTAGG3280.13648752678775772No Hit
GACTACCGGGGTATCTAATCCTGCAGCCCCCCGTAGG3090.12858123712627179No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3030.12608451407527618No Hit
GACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3020.12566839356677695No Hit
GACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2950.1227555500072821No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2890.12025882695628654No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2800.1165137423797932No Hit
GACTACTCGGGTATCTAATCCTGCAGCCACCCGTAGG2790.11609762187129392No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2630.10943969373530575No Hit
GACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA2590.10777521170130869No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2490.10361400661631609No Hit
GACTACTAGGGTATCTAATCCTGCAGCCACCCGTAGG2480.10319788610781683No Hit
GACTACAGGGGTATCTAATCCTGCAGCCACCCGTAGG2420.10070116305682125No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2410.10028504254832199No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2410.10028504254832199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)