FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240619
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT194998.103682585331997No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT167716.9699400296734675No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC142935.9400961686317375No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC125175.201999842073985No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT119664.973007119138555No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT118284.915655039710081No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC94143.9124092444902523No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC86483.5940636441843745No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT78143.247457598942727No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT70592.933683541199988No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT54482.264160353089324No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT48782.027271329363018No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT47341.9674256812637407No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC44581.8527215224067923No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT36291.508193451057481No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC27871.1582626475880957No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT27291.134158150436998No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25391.0551951425282293No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT23050.9579459643669037No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC20410.8482289428515619No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT19260.8004355433278336No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC17030.7077579077296473No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT15500.6441719066241651No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT13710.5697804412785358No Hit
CCTACGGGGGGCTGCAGTTAGGAATCTTCCACAATGGACGAAAGTCTGAT13190.5481695127982412No Hit
CCTACGGGTGGCTGCAGTTAGGAATCTTCCACAATGGACGAAAGTCTGAT11110.46172579887706294No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT10190.4234910792580802No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT8540.35491794081099165No Hit
CCTACGGGAGGCTGCAGTTAGGAATCTTCCACAATGGACGAAAGTCTGAT7860.3266574958752218No Hit
CCTACGGGCGGCTGCAGTTAGGAATCTTCCACAATGGACGAAAGTCTGAT7750.32208595331208256No Hit
CCTACGGGGTGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC7150.2971502666040504No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT6940.28842277625623913No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6530.2713833903390838No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC5440.22608355948615863No Hit
CCTACGGGGGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCCAGTAGTC5330.2215120169230194No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT5090.2115377422398065No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT5030.2090441735690033No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT4940.20530382056279844No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4470.18577086597483988No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG4440.1845240816394383No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4040.16790029050075014No Hit
CCTACGGGTGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCCAGTAGTC3960.16457553227301253No Hit
CCTACGGGGGGCTGCTGGTCCCAGCGCTGACAAGTCTTGCCTGGGATTAGATACCCCAGTAGTC3860.16041958448834048No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3850.16000398970987328No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3310.1375618716726443No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3290.13673068211570988No Hit
CCTACGGTGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT3280.13631508733724268No Hit
CCTACGGGGGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCGAGTAGTC3260.13548389778030828No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3210.13340592388797226No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3170.13174354477410347No Hit
CCTACGGGGGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCCTGTAGTC3080.12800319176789862No Hit
CCTACGGGTGGCTGCTGGTCCCAGCGCTGACAAGTCTTGCCTGGGATTAGATACCCCAGTAGTC3000.124678433540161No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT3000.124678433540161No Hit
CCTACGGGGGGCAGCAGTTAGGAATCTTCCACAATGGACGAAAGTCTGAT2970.1234316492047594No Hit
CCTACGGGCGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCCAGTAGTC2900.12052248575548898No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT2890.12010689097702178No Hit
CCTACGGGGGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCTAGTAGTC2870.11927570142008735No Hit
CCTACGGGAGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCCAGTAGTC2840.11802891708468574No Hit
CCTACGGGGAGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2810.11678213274928413No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2640.10971702151534167No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2600.10805464240147286No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAACCCTGAT2570.10680785806607125No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAC2570.10680785806607125No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT2490.10348309983833363No Hit
CCTACGGGAGGCTGCTGGTCCCAGCGCTGACAAGTCTTGCCTGGGATTAGATACCCCAGTAGTC2450.10182072072446482No Hit
CCTACGGGGGGCTGCTGGTCCCAGCGCTGACAAGTCTTGCCTGGGATTAGATACCCGAGTAGTC2440.1014051259459976No Hit
CCTACGGGTGGCTGCAGTCTGTTTCTAGGTGTGTGCGAGGCAGCTTGCAGCAAGGATTAGATACCCGAGTAGTC2440.1014051259459976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)