FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240619
Sequences flagged as poor quality0
Sequence length35-301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA127825.312132458367793No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG109864.565724236240696No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG89063.701287097028913No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA76253.168910185812426No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA74373.0907783674605915No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA64102.6639625299747736No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG63742.649001117949954No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG63202.6265589999127252No Hit
GACTACTGGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG62572.6003765288692913No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA55672.313616131726921No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG54902.2816153337849463No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG51322.1328324030936874No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG48222.0039980217688544No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG47891.9902833940794369No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA47691.9819714985100927No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA46761.9433211841126428No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA46211.9204634712969466No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG43571.8107464497816048No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA41431.7218091671896234No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG41121.70892572905714No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG38931.6179104725728228No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG37231.5472593602333982No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG37031.538947464664054No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG35061.457075293306015No Hit
GACTACAGGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG33871.4076195146684176No Hit
GACTACTCGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG32641.3565013569169515No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG31351.3028896304946824No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG28771.195666177650144No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG28351.1782111969545215No Hit
GACTACTAGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG27411.1391452877786044No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG27191.130002202652326No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25441.0572731164205653No Hit
GACTACCGGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG22230.9238671925325931No Hit
GACTACACGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG21350.887294852027479No Hit
GACTACCCGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG18980.788798889530752No Hit
GACTACCAGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG18970.7883832947522847No Hit
GACTACAAGGGTATCTAATCCTTTTTGCTCCCCACGCTTTCGCACCTCAG17010.7069267181727129No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG11600.4820899430219559No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA8300.34494366612777877No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG7150.2971502666040504No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG7030.2921631292624439No Hit
GACTACTGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCCCCCGTAGG6170.25642197831426444No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG5750.2389669976186419No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG5510.22899272293542905No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4840.20114787277812643No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG4740.19699192499345436No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG4590.19075800331644632No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG4480.18618646075330708No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4380.18203051296863507No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG4240.1762121860700942No Hit
GACTACTGGGGTATCTAATCCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCCCCCGTAGG4240.1762121860700942No Hit
GACTACTGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCACCCGTAGG4200.1745498069562254No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA4180.17371861739929098No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTACCTCAG3630.1508609045835948No Hit
GACTACTCGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCCCCCGTAGG3570.1483673359127916No Hit
GACTACAGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCCCCCGTAGG3540.14712055157738999No Hit
GACTACTAGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCCCCCGTAGG3260.13548389778030828No Hit
GACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTCA3060.12717200221096422No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG3040.12634081265402983No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA3020.1255096230970954No Hit
GACTACTGGGGTATCTAATCCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCACCCGTAGG3010.1250940283186282No Hit
GACTACTGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCGCCCGTAGG2940.12218486486935778No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2900.12052248575548898No Hit
GACTACTGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCTCCCGTAGG2870.11927570142008735No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2730.11345737452154651No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2710.1126261849646121No Hit
GACTACTCGGGTATCTAATCCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCCCCCGTAGG2590.10763904762300566No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2570.10680785806607125No Hit
GACTACTCGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCACCCGTAGG2560.10639226328760405No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2510.10431428939526805No Hit
GACTACTGGGGTATCTAATCCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCTCCCGTAGG2510.10431428939526805No Hit
GACTACTAGGGTATCTAATCCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCCCCCGTAGG2490.10348309983833363No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTCA2490.10348309983833363No Hit
GACTACTGGGGTATCTAATCCCAGGCAAGACTTGTCAGCGCTGGGACCAGCAGCCGCCCGTAGG2480.10306750505986643No Hit
GACTACCGGGGTATCTAATCCTTGCTGCAAGCTGCCTCGCACACACCTAGAAACAGACTGCAGCCCCCCGTAGG2460.10223631550293202No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2430.10098953116753041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)