FastQCFastQC Report
Fri 4 Aug 2023
EGAF00002486787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002486787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238365
Sequences flagged as poor quality0
Sequence length35-301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC103594.345856144987729No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT99714.183080569714513No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC96554.050510771296121No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT86983.6490256539340926No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC77653.257609128857005No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC71242.9886938099133684No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC68512.8741635726721624No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT63192.6509764436893No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT62022.601892056300212No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC61512.5804962976947117No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC50722.127829169550899No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC49212.0644809430914774No Hit
CCTACGGGGGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC46741.9608583474922912No Hit
CCTACGGGTGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC43151.8102489878967132No Hit
CCTACGGGCGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC33821.4188324628196254No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT29671.244729721225851No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC28231.1843181675162042No Hit
CCTACGGGAGGCTGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC28071.1776057726595768No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC25631.0752417510960082No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC21980.9221152434291948No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC20890.8763870534684203No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT20850.8747089547542634No Hit
CCTACGGGGGGCTGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC20790.8721918066830281No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT20170.8461812766135968No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT19680.8256245673651753No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19380.8130388270089988No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC18820.7895454450108027No Hit
CCTACGGGTGGCTGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC17570.7371048601934008No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC16070.6741761584125187No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC15790.6624294674134206No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT15500.6502632517357834No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT15260.6401946594508422No Hit
CCTACGGGGGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC14600.612506030667254No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT14170.5944664694900678No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14080.5906907473832148No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC13510.5667778407064796No Hit
CCTACGGGCGGCTGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC12910.5416063599941267No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12580.5277620456023325No Hit
CCTACGGGAGGCTGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC12190.5114005831393031No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC11360.47658003482054834No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC10280.4312713695383131No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9260.38847985232731314No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC8980.3767331613282151No Hit
CCTACGGGTGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC8940.37505506261405824No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC8580.35995217418664655No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC8010.33603926750991125No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC7880.3305854466889015No Hit
CCTACGGGGTGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC7140.2995406204769996No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT6920.2903110775491368No Hit
CCTACGGGGGGCAGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC6810.28569630608520546No Hit
CCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6550.2747886644431859No Hit
CCTACGGGCGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC6220.26094435005139177No Hit
CCTACGGGGGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG6190.25968577601577414No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC6170.2588467266586957No Hit
CCTACGGGGGGCTGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC5850.24542193694544082No Hit
CCTACGGGTGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG5550.23283619658926438No Hit
CCTACGGGTGGCTGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC5440.228221425125333No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC5280.22150903026870555No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT5110.21437711073353888No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4980.2089232899125291No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4700.1971765989134311No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC4590.1925618274494997No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4550.19088372873534287No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4280.17955656241478404No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT4250.1782979883791664No Hit
CCTACGGGTGGCAGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC4180.1753613156293919No Hit
CCTACGGGAGGCTGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC3970.16655129738006838No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA3930.16487319866591152No Hit
CCTACGGGCGGCTGCAGCTAAGAATATTCCGCAATGGACGAAAGTCTGAC3880.16277557527321546No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3870.16235605059467623No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA3750.15732175445220567No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3690.15480460638097035No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3560.14935078555996056No Hit
CCTACGGGCGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3440.14431648941748998No Hit
CCTACGGGAGGCTGCAGTGATAAACCTTTAGCAATAAACCAAAGTTTAAG3390.1422188660247939No Hit
CCTACGGGGAGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3320.1392821932750194No Hit
CCTACGGGAGGCAGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC3190.1338283724540096No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3090.12963312566861745No Hit
CCTACGGGGGGCTGCAAGGATTAGATACCCCAGTAGTC3050.1279550269544606No Hit
CCTACGGGGAGCTGCAGTGGGGAATCTTGCGCAATGGGCGAAAGCCTGAC2910.12208168145491159No Hit
CCTACGGGCGGCAGCAGTAACGAATCTTCCGCAATGCACGAAAGTGTGAC2880.12082310741929395No Hit
CCTACGGGGGGCAGCAGTGATAAACCTTTAGCAATAAACGAAAGTTTAAC2800.11746690999098021No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA2740.11494976191974493No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2680.11243261384850964No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGAA2410.10110544752795084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)