FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002487211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55964205
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG14223512.5415370413999447TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG1854830.3314314926835823TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1340360.23950308951945265TruSeq Adapter, Index 12 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG567910.1014773639686296TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)