FastQCFastQC Report
Wed 14 Sep 2022
EGAF00002487219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38836461
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1040577726.793834278566216TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG9716002.5017727542167143TruSeq Adapter, Index 4 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1165550.30011745920927246No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG914590.23549777102501693TruSeq Adapter, Index 4 (98% over 63bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG758250.19524178580535442TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG751680.19355007656336143TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG707070.18206344805722643TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG665600.17138533812336815TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG543500.13994581020139812TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG487720.1255830184938839TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG477110.12285104968756036TruSeq Adapter, Index 4 (100% over 63bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG440220.11335224391326491TruSeq Adapter, Index 4 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)