FastQCFastQC Report
Sat 13 May 2023
EGAF00002487221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39984571
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG22643635.663091896121632TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1345160.33641976551405295TruSeq Adapter, Index 5 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG764440.19118374434978933TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG463710.11597223338972425TruSeq Adapter, Index 5 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG436470.10915960558886577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)