FastQCFastQC Report
Sat 13 May 2023
EGAF00002487229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41494800
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG23464915.654903746975524TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG615820.14840895726693465TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGG461390.11119224577537427TruSeq Adapter, Index 8 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)