FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541253
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA25780.4763022098722779No Hit
CCTCCCACTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19460.35953611342569924No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT19450.35935135694398No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19040.3517763411934899No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA16170.29875123094005945No Hit
GGCACAGACCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGT14700.2715920281273268No Hit
CATTTAAACCCCACTAAAACTAAACGTTGGGGTTACTCCCTTAACTTCAT13300.24572612068662897No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAACACC13100.2420309910522436No Hit
GAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGAGA12990.23999866975333162No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT12360.22835901140501763No Hit
CCAATTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACT11360.20988336323309065No Hit
CCTCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGG10960.20249310396431983No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA10810.1997217567385308No Hit
CCTAAACATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG10350.19122295857944435No Hit
GCGTTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC10220.18882112431709386No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10220.18882112431709386No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA10210.18863636783537457No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA10100.18660404653646262No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC10070.1860497770913048No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA9930.18346318634723502No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9720.17958330023113037No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG9680.1788442743042533No Hit
AGCGTTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA9610.1775509789322184No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9500.1755186576333064No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9290.17163877151720175No Hit
GTATCGGGAGGCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9250.17089974559032467No Hit
CCTAACGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8970.16572656410218511No Hit
ATATGATCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8710.1609228955774841No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8500.15704300946137942No Hit
GTAGACGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT8400.15519544464418672No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTT8170.15094604556464353No Hit
GGATCATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA8090.14946799371088937No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTTACTACTAATTCCCTGG8060.14891372426573155No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7940.1466966464851003No Hit
TAGTCAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAG7880.1455881075947847No Hit
GTCTTTGCCAAGTGTTTGCGGATGCAACCCCCACTGGTTGGGGCTTGGCC7830.14466432518618835No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7820.14447956870446907No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG7810.14429481222274979No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7740.1430015168507149No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT7510.13875211777117172No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7440.13745882239913682No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7290.13468747517334775No Hit
CCAGTTGGACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACT7200.13302466683787434No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT7110.13136185850240092No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7090.13099234553896238No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG7050.13025331961208528No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA7030.12988380664864674No Hit
CGCTTGATGTGTCGGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT6940.12822099831317332No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6890.12729721590457696No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6770.12508013812394572No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6760.12489538164222645No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6650.12286306034331448No Hit
GACTTCTTTCCTTCTATTCGTGATCTCCTCGACACCGCCTCTGCCCTGTA6600.12193927793471815No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6560.12120025200784107No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6380.1178746353368942No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6370.11768987885517493No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6320.1167660964465786No Hit
CCTGTCGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA6320.1167660964465786No Hit
ATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGT6290.11621182700142078No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6260.11565755755626296No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6260.11565755755626296No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6250.11547280107454369No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6230.11510328811110515No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6220.11491853162938588No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAATGTCTTGTAAGTTGGCGA6210.11473377514766663No Hit
ATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA6090.11251669736703537No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5970.11029961958640414No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5930.10956059365952707No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5920.1093758371778078No Hit
CTTCTATTCGTGATCTCCTCGACACCGCCTCTGCCCTGTATCGGGAGGCC5870.10845205476921144No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5810.10734351587889583No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCA5810.10734351587889583No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5800.10715875939717656No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5790.10697400291545729No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT5770.10660448995201874No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5730.10586546402514166No Hit
GTGTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5730.10586546402514166No Hit
GGCAAAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGT5710.10549595106170312No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5690.1051264380982646No Hit
GTTAAGGGAGTAACCCCAACGTTTAGTTTTAGTGGGGTTTAAATGTATAC5640.10420265568966823No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5640.10420265568966823No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5610.10364838624451042No Hit
GCCCTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5590.10327887328107188No Hit
GATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCT5580.10309411679935261No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT5520.10198557790903699No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5500.10161606494559845No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5480.10124655198215991No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACA5470.10106179550044064No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5470.10106179550044064No Hit
CCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACTTGTATTCC5470.10106179550044064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)