FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316088
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14500.458733011060211No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT14270.45145655640201465No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14180.448609248057503No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11470.3628736301283187No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11170.35338260231327984No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8900.2815671585128192No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7950.25151223709852955No Hit
GATTAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTG7700.2436030472526638No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT7640.24170484168965606No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG7540.2385411657513098No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7400.234112019437625No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC6940.21955911012123205No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6710.2122826554630356No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6060.19171876186378475No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5980.18918782111310775No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5960.1885550859254385No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG5810.18380957201791906No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5790.1831768368302498No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5730.18127863126724203No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5520.17463491179671484No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5470.1730530738275417No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT5470.1730530738275417No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5300.167674824732353No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5280.16704208954468375No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5070.16039837007415658No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5060.16008200248032192No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5050.1597656348864873No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4950.15660195894814102No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4910.1553364885728025No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4850.15343828300979473No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4750.15027460707144846No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4710.14900913669610993No Hit
ATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC4700.14869276910227533No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4690.1483764015084407No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4650.14711093113310217No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4320.13667080053655944No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4300.13603806534889018No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA4300.13603806534889018No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4230.13382349219204778No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4200.1328743894105439No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC4190.13255802181670928No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4180.13224165422287465No Hit
CTGTTGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGCTGT4150.13129255144137075No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA4050.12812887550302446No Hit
GATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC4020.1271797727215206No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT3970.12559793475234746No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATT3950.12496519956467819No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA3900.12338336159550506No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC3890.12306699400167043No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG3870.12243425881400118No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3860.12211789122016654No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG3850.1218015236263319No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3820.12085242084482803No Hit
TGGAAACCACCGTGAACGCCCGCCAAGTCTTGCCCAAGGTCTTACATAAG3800.12021968565715878No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA3770.1192705828756549No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3770.1192705828756549No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3770.1192705828756549No Hit
GCACTAAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3710.1173723773126471No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3690.11673964212497785No Hit
CATACTGCGGAACTCCTAGCAGCATGTTTTGCTCGCAGCCGGTCTGGAGC3690.11673964212497785No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC3680.11642327453114322No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3660.11579053934347397No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG3650.11547417174963934No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3630.11484143656197009No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3600.1138923337804662No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3590.11357596618663157No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3580.11325959859279694No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTC3580.11325959859279694No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3550.11231049581129306No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA3470.10977955506061603No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTTACTACTAATTCCCTGG3420.1081977170914429No Hit
CCTTCTCGGCACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT3380.10693224671610438No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC3360.10629951152843513No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3350.1059831439346005No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG3320.10503404115309661No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC3310.10471767355926197No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG3280.10376857077775808No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG3270.10345220318392345No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3260.10313583559008885No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3240.10250310040241957No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG3230.10218673280858497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)