FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473755
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA20860.4403119755992021No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19400.4094943588985868No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18460.3896528796529852No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17580.371077877806039No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13360.2820023007672742No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA12410.26194974195523No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT11240.2372534326814493No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10760.22712161349220586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10700.22585513609355046No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT10310.21762303300229022No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG10050.2121349642747834No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG9440.1992591107217866No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT9210.19440428069360746No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG8910.18807189370033034No Hit
AATTTGAGGACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCAAAT8320.17561819928021866No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG8310.1754071197137761No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT8240.1739295627486781No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT8140.1718187670842524No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8130.17160768751780983No Hit
CCTTTACCCTGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7920.16717501662251585No Hit
GTGTAAACAATATCTGAACCTTTACCCTGTTGCTCGGCAACGGTCAGGTC7910.1669639370560733No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7500.1583096748319279No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7450.15725427699971503No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7420.15662103830038734No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA7320.15451024263596164No Hit
GGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCC7270.15345484480374877No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7170.15134404913932306No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCGCCACCAATCGGCAGT7170.15134404913932306No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7120.15028865130711022No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATGACATATCCCATGAA7110.15007757174066763No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7080.14944433304133994No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7070.14923325347489738No Hit
GCATTAATGCCTTTGTATGCATGTATTCAAGCTAAACAGGCTTTCACTTT7030.14838893520912708No Hit
CTTCTCGGCACCGACAACTCTGTTGTCCTCTCTCGAAAATACACCTCCTT6940.14648921911114393No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6890.1454338212789311No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT6820.1439562643138331No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6720.1418454686494074No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6690.14121222995007968No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6650.1403679116843094No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6630.13994575255142425No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6590.13910143428565397No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC6480.1367795590547857No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6290.13276904729237685No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTTCGAGAGAGGACAACAGAGT6230.13150256989372144No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6210.1310804107608363No Hit
GGTTGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC6170.130236092495066No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6170.130236092495066No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6110.12896961509641058No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6110.12896961509641058No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATTATGCCTGCTAGG6100.12875853552996802No Hit
CTCGAAAATACACCTCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG6100.12875853552996802No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5950.12559234203332945No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT5900.1245369442011166No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5890.12432586463467403No Hit
ATCTGAACCTTTACCCTGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5880.12411478506823147No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5870.1239037055017889No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT5850.12348154636890377No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5830.12305938723601861No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5680.11989319373938007No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5660.11947103460649491No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5580.11778239807495436No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5530.1167270002427415No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5470.11546052284408607No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5400.1139829658789881No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG5400.1139829658789881No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5340.11271648848033267No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT5330.11250540891389008No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5320.11229432934744751No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATTATG5320.11229432934744751No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5320.11229432934744751No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5290.11166109064811981No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5280.11145001108167725No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG5270.11123893151523466No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5210.10997245411657923No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5100.10765057888571096No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC5090.1074394993192684No Hit
TTAGCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC5020.1059619423541704No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4990.1053287036548427No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC4980.10511762408840011No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGGTA4910.10364006712330212No Hit
GCCGATAAGTGTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGA4880.10300682842397442No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4830.10195143059176157No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC4830.10195143059176157No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT4820.101740351025319No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAACCCCTGTTCCGA4810.10152927145887641No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4770.10068495319310615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)