FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550415
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22710.4125977671393403No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19760.35900184406311597No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTATATGCATGTAT17250.31339988917453193No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC13850.25162831681549375No Hit
GTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAATTCTTTGACATACT13660.24817637600719455No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12300.2234677470635793No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12170.22110589282632195No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12140.22056084954080102No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11430.20766149178347246No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT11120.20202937783308955No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10500.1907651499323238No Hit
GCCCACTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAAT10140.18422463050607268No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9970.1811360518881208No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACACAG9770.17750242998464796No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACAC9670.17568561903291152No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGATCT9460.17187031603426506No Hit
GGGACTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTT9440.17150695384391776No Hit
GGACTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTTG9240.16787333194044496No Hit
GGTCCTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC9010.16369466675145117No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA8910.16187785579971475No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8830.1604244070383256No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8810.16006104484797834No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8330.15134035227964354No Hit
GTTCAACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAA8300.1507953089941226No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC8020.14570823832926066No Hit
GTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGC8020.14570823832926066No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT7890.14334638409200331No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7880.14316470299682965No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7850.14261965971130872No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC7810.14189293533061417No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7710.14007612437887776No Hit
GCCACAAAGCTTCCACGCATGCGCTGATGGCCCATGGCCAAGCCCCAACC7700.13989444328370412No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT7640.13880435671266225No Hit
CTTCTGGACTACCGAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC7630.13862267561748864No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7620.13844099452231498No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7560.1373509079512731No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7560.1373509079512731No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7530.1368058646657522No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7470.13571577809471036No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATGGATAATATCCTCTTTT7450.1353524159043631No Hit
GGATATTATCCATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7310.1328088805719321No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7300.13262719947675844No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7290.1324455183815848No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT7280.13226383728641117No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT7150.12990198304915382No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGGTCATGCCTGCTAGG7140.12972030195398018No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAGATTACTTCCCACCC7090.12881189647811198No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG7080.12863021538293834No Hit
GTGGATACCCAGCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAG6980.1268134044312019No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6910.12554163676498642No Hit
ATGACTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGG6900.12535995566981276No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG6850.12445155019394456No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6810.12372482581325No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6700.12172633376633996No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6660.12099960938564537No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6650.12081792829047175No Hit
CTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTCAGGATTAAAG6450.11718430638699891No Hit
GGTTAGGATAGAACCTAGCAGGCATGACCAATTTTAATCTCCTTTTTTCG6410.11645758200630432No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6210.11282396010283151No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6200.11264227900765786No Hit
GTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6200.11264227900765786No Hit
GTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGG6200.11264227900765786No Hit
GGGTATCCACATTGTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAAT6070.11028042477040051No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5940.10791857053314317No Hit
GATTTTTAGGCCCGTATTAACATTGACATAGCTTACTACTAATTCCCTGG5940.10791857053314317No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5890.10701016505727497No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5880.10682848396210132No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5770.10482999191519127No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5770.10482999191519127No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT5760.10464831082001762No Hit
CCTCGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5750.10446662972484397No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5740.10428494862967035No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5680.10319486205862848No Hit
TGCAAGACCTGCACGAGTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5650.10264981877310757No Hit
TGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5640.10246813767793392No Hit
TACCTCGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5600.10174141329723936No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5590.10155973220206571No Hit
TCTATGGATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTT5580.10137805110689206No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5570.10119637001171844No Hit
GGTACGGTTAGGATAGAACCTAGCAGGCATGACCAATTTTAATCTCCTTT5520.10028796453585022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)