FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550415
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA20460.3717195207252709No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16710.30358911003515526No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTATATGCATGTAT15940.2895996657067849No Hit
GTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAATTCTTTGACATACT13980.2539901710527511No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC13560.24635956505545814No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13250.24072745110507526No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT10850.19712398826340125No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10560.19185523650336564No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10010.18186277626881533No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10000.1816810951736417No Hit
GGACTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTTG9580.17405048917634877No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9490.17241535931978597No Hit
GCCCACTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAAT8950.16260458018040932No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8800.1598793637528047No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8790.15969768265763107No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGATTGAGATCT8720.15842591499141556No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8460.1537022065169009No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8270.15025026570860167No Hit
GTTCAACTGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTATAAA8130.14770673037617071No Hit
GGGACTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTT8120.14752504928099705No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7980.14498151394856607No Hit
GGTCCTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC7760.14098452985474597No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7730.14043948656922503No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG7620.13844099452231498No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA7430.1349890537140158No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT7420.13480737261884215No Hit
CTTCTGGACTACCGAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC7410.13462569152366852No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7360.1337172860478003No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGGTCATGCCTGCTAGG7100.1289935775732856No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7090.12881189647811198No Hit
CCTCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG7000.1271767666215492No Hit
GTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGC6980.1268134044312019No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6960.12645004224085463No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAGATTACTTCCCACCC6940.12608668005050735No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6880.12499659347946548No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATGGATAATATCCTCTTTT6860.12463323128911821No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6780.12317978252772907No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6740.12245305814703451No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6720.12208969595668723No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG6720.12208969595668723No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6680.12136297157599266No Hit
GCCACAAAGCTTCCACGCATGCGCTGATGGCCCATGGCCAAGCCCCAACC6670.12118129048081902No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6660.12099960938564537No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6610.12009120390977716No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6600.11990952281460353No Hit
GGATATTATCCATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6550.11900111733873532No Hit
CTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTCAGGATTAAAG6530.11863775514838805No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6420.11663926310147797No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC6400.1162759009111307No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6400.1162759009111307No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT6340.11518581434008883No Hit
GGTTAGGATAGAACCTAGCAGGCATGACCAATTTTAATCTCCTTTTTTCG6290.11427740886422062No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6250.11355068448352607No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6190.11246059791248421No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6190.11246059791248421No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6180.11227891681731057No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6160.1119155546269633No Hit
GTGGATACCCAGCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAG6140.11155219243661602No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACAC6080.11046210586557416No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6050.10991706258005322No Hit
ATGACTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGG5970.1084636138186641No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5890.10701016505727497No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT5840.10610175958140676No Hit
GATTTTTAGGCCCGTATTAACATTGACATAGCTTACTACTAATTCCCTGG5810.10555671629588582No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5780.1050116730103649No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5770.10482999191519127No Hit
TGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5750.10446662972484397No Hit
GGGTATCCACATTGTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAAT5710.10373990534414941No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5640.10246813767793392No Hit
GTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC5580.10137805110689206No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5570.10119637001171844No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5530.10046964563102387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)