FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414697
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22000.530507816550397No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT19160.4620240802320731No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17690.426576512489842No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC17270.4164486359920617No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14360.34627692025744095No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG13230.31902810968007966No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12840.30962365293214084No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTCTGTGGGTCACCATATT10740.2589842704432393No Hit
CCCTAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGAGCCCA9850.23752281786460958No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9410.22691266153360162No Hit
CTATATAAGAGAGAAACTACTCGCAGCGCCTCATTCTGTGGGTCACCATA9080.21895504428534568No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8980.2165436451192075No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACT8910.21485566570291081No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8840.2131676862866141No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG8780.21172084678693115No Hit
CTTTTAGGGTGGAGCCCTCAGGCTCAGGGCATATTGACCACAGTACCAGC8620.20786260812111013No Hit
ATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC8390.20231639003899232No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8340.20111069045592325No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8270.19942271103962653No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8140.1962878921236469No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG8130.1960467522070331No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7960.1919473736245982No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG7810.18833027487539095No Hit
GCCTAGCAGCCATGGGAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT7530.18157835721020407No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7470.18013151771052116No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7460.17989037779390735No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7390.17820239837761065No Hit
ATCCTAAACTTACCAAATATTTACCCTTAGATAAAGGCATCAAACCTTAT7330.17675555887792774No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT7140.17217390046226522No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7130.1719327605456514No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7100.17120934079580996No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG7010.1690390815462856No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6980.16831566179644414No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6800.16397514329739546No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6720.16204602396448492No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6720.16204602396448492No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG6560.15818778529866384No Hit
GTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6540.1577055054654362No Hit
TTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACTC6530.1574643655488224No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6490.15649980588236714No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6470.1560175260491395No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6380.15384726679961513No Hit
CTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTCAGGATTAAAG6370.15360612688300132No Hit
GCTTACTACTAATTCCCTGGATGAAGGATCTTCCAAATTACTTCCCACCC6220.14998902813379406No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6110.14733648905104207No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATCGA6080.14661306930120063No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGTC6070.14637192938458682No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6010.14492508988490393No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAACCTGGCCAC6000.1446839499682901No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5980.14420167013506247No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5960.14371939030183484No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC5940.1432371104686072No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5910.14251369071876574No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5880.1417902709689243No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT5840.14082571130246904No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5830.1405845713858552No Hit
GCCTGAGGATGACTGTCTCTTAAAGGTGGAGAGATGGGAGTAGGCTGTCT5800.13986115163601376No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5800.13986115163601376No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5750.13865545205294466No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5730.13817317221971706No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5660.13648519280342034No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5590.1347972133871236No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5520.13310923397082688No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5510.13286809405421307No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5510.13286809405421307No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTC5440.13118011463791635No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5420.13069783480468872No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5420.13069783480468872No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTA5420.13069783480468872No Hit
ATCTAAGGGTAAATATTTGGTAAGTTTAGGATAGAACCTAGCAGGCATGA5410.1304566948880749No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT5400.1302155549714611No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5380.12973327513823346No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAACC5370.12949213522161965No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC5370.12949213522161965No Hit
ATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGACTCTACCGTCCCCTT5370.12949213522161965No Hit
CCCTTGGACACATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5350.129009855388392No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5340.12876871547177818No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACAACGAGGC5290.1275630158887091No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5290.1275630158887091No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5280.1273218759720953No Hit
CAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGA5240.12635731630564No Hit
GTACAAAGATCATTAACCTGGTCTCCTCCCCCAACTCCTCCCAGTCCTTA5210.12563389655579857No Hit
TCATTAACCTGGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACACAG5210.12563389655579857No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5200.12539275663918475No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5140.12394591713950184No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5100.12298135747304659No Hit
GTCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC4960.11960539864045315No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4950.11936425872383932No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4910.11839969905738407No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4900.11815855914077025No Hit
GTGTTGGGGTGAGTTAATGAACCTGGCCACCTGGGTGGGAAGTAATTTGG4880.11767627930754261No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4850.11695285955770117No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTG4850.11695285955770117No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTAAGTTT4830.11647057972447353No Hit
CCTTTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCAGCACATT4820.11622943980785971No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4750.11454146039156299No Hit
GTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCACCTTATGTGTCCAA4740.11430032047494917No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4720.11381804064172156No Hit
GTGGTGGAGTGAACCCCGGCCCGAATGAGCCCACTCCTACCTGGTTTGCC4720.11381804064172156No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA4710.11357690072510773No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGAAGGATCTTCC4700.11333576080849392No Hit
GGTTGGGGCTTGGCCATGGACCATCAGCGCATGCGTGGAACCTTTGTGGC4650.11213006122542483No Hit
GCTGGTACTGTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCTAAAAG4650.11213006122542483No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4580.11044208180912812No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4580.11044208180912812No Hit
CTCCTACCTGGTTTGCCGCTGGCCATTGATCCTTGCTGGGGTTGAAGTCC4580.11044208180912812No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC4540.10947752214267284No Hit
CCTTATGTGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4500.10851296247621758No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4470.10778954272637611No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT4460.1075484028097623No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4450.1073072628931485No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4430.10682498305992086No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAACCTGGTCTCCTCC4420.10658384314330704No Hit
GGTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTG4390.10586042339346557No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAACCTG4390.10586042339346557No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4380.10561928347685179No Hit
TACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACTCGC4360.10513700364362415No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC4290.10344902422732742No Hit
ATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACAACGAGGCAGGAC4280.10320788431071361No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4260.10272560447748597No Hit
GTCCTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4250.10248446456087215No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4250.10248446456087215No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4250.10248446456087215No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4240.10224332464425835No Hit
GACAAAGGACGTCCCGCGTAGGATCCAGTTGGCAGCACAGCCTAGCAGCC4230.10200218472764452No Hit
GGCCTATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT4220.10176104481103071No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4210.10151990489441688No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCTAAAAGATTGCCGTG4200.10127876497780307No Hit
AGATAGGGGCATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACT4190.10103762506118924No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4170.10055534522796163No Hit
CTTATCAACACTTCCGGAAACTACTGTTGTTAGACAACGAGGCAGGACCC4160.10031420531134781No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4160.10031420531134781No Hit
ATTCTGTGTTGGGGTGAGTTAATGAACCTGGCCACCTGGGTGGGAAGTAA4160.10031420531134781No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4150.10007306539473398No Hit
CTCTTAAAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG4150.10007306539473398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)