FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414697
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19190.46274749998191456No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT17770.4285056318227525No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16950.40873215866041956No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15470.37304345100157466No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC14370.3465180601740548No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG13840.3337376445935225No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTCTGTGGGTCACCATATT13410.32336862817912837No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12070.2910558793528769No Hit
CTATATAAGAGAGAAACTACTCGCAGCGCCTCATTCTGTGGGTCACCATA10650.25681401119371494No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACT10630.25633173136048726No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9130.22016074386841475No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8910.21485566570291081No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8770.21147970687031736No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8230.19845815137317124No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG8220.19821701145655746No Hit
CTTTTAGGGTGGAGCCCTCAGGCTCAGGGCATATTGACCACAGTACCAGC7990.19267079337443965No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7980.19242965345782584No Hit
ATCCTAAACTTACCAAATATTTACCCTTAGATAAAGGCATCAAACCTTAT7590.18302519670988698No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG7550.1820606370434317No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG7460.17989037779390735No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG7390.17820239837761065No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7290.17579099921147248No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7220.17410301979517576No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7110.17145048071242375No Hit
TTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACTC7110.17145048071242375No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7110.17145048071242375No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7070.1704859210459685No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6970.16807452187983032No Hit
GCCTAGCAGCCATGGGAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6910.1666276823801474No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT6890.1661454025469198No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6880.165904262630306No Hit
GTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6730.16228716388109873No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6660.160599184464802No Hit
CTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTCAGGATTAAAG6640.16011690463157438No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6630.15987576471496054No Hit
ATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC6510.15698208571559474No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6460.15577638613252567No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6460.15577638613252567No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATCGA6440.15529410629929805No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6410.1545706865494566No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC6330.15264156721654606No Hit
ATCCCGCGGACGACCCGTCTCGGGGCCGTTTGGGACTCTACCGTCCCCTT6230.15023016805040787No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6210.14974788821718027No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6170.14878332855072499No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6070.14637192938458682No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6070.14637192938458682No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6010.14492508988490393No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5770.13913773188617232No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5770.13913773188617232No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5710.1376908923864894No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAACC5700.1374497524698756No Hit
CTCCTACCTGGTTTGCCGCTGGCCATTGATCCTTGCTGGGGTTGAAGTCC5640.1360029129701927No Hit
ATCTAAGGGTAAATATTTGGTAAGTTTAGGATAGAACCTAGCAGGCATGA5610.13527949322035124No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5590.1347972133871236No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5470.13190353438775781No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5420.13069783480468872No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5400.1302155549714611No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5390.12997441505484728No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5380.12973327513823346No Hit
CCCTTGGACACATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5360.12925099530500583No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC5320.12828643563855055No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAACCTGGCCAC5300.12780415580532292No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5280.1273218759720953No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5260.12683959613886767No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGTC5250.12659845622225385No Hit
GCTTACTACTAATTCCCTGGATGAAGGATCTTCCAAATTACTTCCCACCC5210.12563389655579857No Hit
GCCTGAGGATGACTGTCTCTTAAAGGTGGAGAGATGGGAGTAGGCTGTCT5170.1246693368893433No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTG5140.12394591713950184No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5120.12346363730627423No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5110.1232224973896604No Hit
CCTTATGTGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5090.12274021755643277No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTCTA5060.12201679780659133No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5040.12153451797336369No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5000.1205699583069084No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT4990.1203288183902946No Hit
CCTTTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCAGCACATT4990.1203288183902946No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4990.1203288183902946No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4960.11960539864045315No Hit
GTGTTGGGGTGAGTTAATGAACCTGGCCACCTGGGTGGGAAGTAATTTGG4920.1186408389739979No Hit
GTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCACCTTATGTGTCCAA4920.1186408389739979No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4890.11791741922415643No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4890.11791741922415643No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTAAGTTT4850.11695285955770117No Hit
CTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACTCGCAGCGCC4830.11647057972447353No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4820.11622943980785971No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4810.1159882998912459No Hit
GTCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC4790.11550602005801827No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4790.11550602005801827No Hit
CAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGA4780.11526488014140446No Hit
GGTTGGGGCTTGGCCATGGACCATCAGCGCATGCGTGGAACCTTTGTGGC4770.11502374022479063No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4750.11454146039156299No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4720.11381804064172156No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4710.11357690072510773No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACAACGAGGC4630.11164778139219719No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4570.1102009418925143No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT4540.10947752214267284No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGAAGGATCTTCC4530.10923638222605903No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA4480.10803068264298994No Hit
GGTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTG4460.1075484028097623No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4450.1073072628931485No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4420.10658384314330704No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTA4390.10586042339346557No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4380.10561928347685179No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4370.10537814356023796No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTC4360.10513700364362415No Hit
GGCCTATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT4350.10489586372701032No Hit
CCCTAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGAGCCCA4330.10441358389378268No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4320.10417244397716886No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4280.10320788431071361No Hit
TACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACTCGC4220.10176104481103071No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4220.10176104481103071No Hit
GTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACTCGCAGCGCCTCAT4180.10079648514457543No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT4160.10031420531134781No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4150.10007306539473398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)