FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366937
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24350.6636016536898705No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17090.4657475261420898No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA12860.3504688815791267No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11670.31803824634746564No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG11450.31204266672480563No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11410.3109525613388674No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11100.3025042445978465No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10520.2866977165017428No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9870.2689835039802473No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8260.22510676219623535No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8250.22483423584975076No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATGACATATCCCATGAA8170.2226540250778744No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8040.2191111825735753No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA7840.2136606556438844No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7690.20957276044661619No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG7590.20684749698177074No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7460.2033046544774716No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7420.20221454909153344No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7420.20221454909153344No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGACC7340.20003433831965708No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6910.18831570542082154No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6650.18123002041222333No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6600.17986738867980062No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6560.17877728329386244No Hit
GAAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG6500.17714212521495515No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6380.1738718090571406No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6220.16951138751338787No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG6220.16951138751338787No Hit
CTGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC6170.16814875578096514No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTAATCTCTTTTTTGCCTTCTG6150.16760370308799602No Hit
TTCAAGAACAGTGTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA6110.16651359770205784No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6060.1651509659696351No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6030.16433338693018149No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6020.16406086058369693No Hit
GTACATTCCTGCTGGTGGCTCCAGTTCAGAAACAGTGAGCCCTGCTCAGA5990.16324328154424328No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5970.1626982288512742No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5960.16242570250478966No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5790.15779275461455236No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5720.15588507018916054No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5670.15452243845673783No Hit
GCACCGGACCATGCAAGACCTGCACAACTCCTGCTCAAGGAACCTCTATG5670.15452243845673783No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5530.15070706960595415No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5520.1504345432594696No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTAAGA5510.15016201691298506No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5500.14988949056650053No Hit
ATTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAATGT5450.1485268588340778No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5390.1468917007551705No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5360.14607412171571688No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5350.14580159536923232No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5330.14525654267626323No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5330.14525654267626323No Hit
ACACAGAATAGCTTGCCTGATTGCCGTATGGTGAGGTGAACAATGTTCCG5300.14443896363680958No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5260.1433488582508714No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5250.14307633190438687No Hit
GTTCTTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC5240.1428038055579023No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5210.1419862265184487No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5200.14171370017196414No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5090.13871591036063413No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5080.13844338401414957No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5040.1373532786282114No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5010.13653569958875775No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4980.1357181205493041No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4930.13435548881688136No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4870.1327203307379741No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4860.13244780439148954No Hit
CCCAATGACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4850.132175278045005No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4840.13190275169852045No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4830.13163022535203592No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4770.12999506727312862No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4730.12890496188719044No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4700.1280873828477368No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4660.12699727746179862No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4620.12590717207586044No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA4560.12427201399695315No Hit
GGAATGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4530.12345443495749953No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4530.12345443495749953No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4490.12236432957156135No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4460.12154675053210769No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTCTTGCATGGTCCGGTG4440.12100169783913861No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT4390.11963906610671586No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4370.11909401341374677No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4340.11827643437429312No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4340.11827643437429312No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT4330.1180039080278086No Hit
GACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTATGAG4320.11773138168132405No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG4310.11745885533483949No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACAG4270.11636874994890131No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGA4260.11609622360241677No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG4260.11609622360241677No Hit
CCCTTAACTTCATGGGATATGTCATTGGGAGTTGGGGCACATTGCCACAG4260.11609622360241677No Hit
GTCATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT4250.11582369725593221No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4230.11527864456296313No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGAC4220.11500611821647858No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4200.11446106552350949No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4190.11418853917702493No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4190.11418853917702493No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4150.11309843379108675No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4120.11228085475163312No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC4110.11200832840514857No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4110.11200832840514857No Hit
GGGTAAAGGTTCAGATACTGTTTACAGAGAAAGGTCTTGTAAGTTGGCGA4040.11010064397975673No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4020.10955559128678766No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4010.10928306494030311No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCT4000.10901053859381855No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA3990.10873801224733401No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC3980.10846548590084945No Hit
CCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTAAGACA3960.10792043320788038No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC3930.10710285416842673No Hit
ATCCAGCATCCAGGGAATTAGTCGTCGGCTATGTCAACGTTAATATGGGC3920.1068303278219422No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG3920.1068303278219422No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGGTTT3860.10519516974303492No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA3850.10492264339655036No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3830.10437759070358126No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3820.10410506435709674No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG3810.10383253801061218No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG3800.10356001166412764No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG3800.10356001166412764No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC3750.1021973799317049No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3750.1021973799317049No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA3710.10110727454576672No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC3690.10056222185279762No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG3680.10028969550631306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)