FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366937
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20660.5630394318370728No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14670.39979615029282956No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA13370.3643677252498385No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG12090.3294843528998166No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10860.29596361228221735No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9430.25699234473492727No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9310.2537220285771127No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9220.25126929145875176No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG9070.24718139626148358No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9030.2460912908755454No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATGACATATCCCATGAA8640.2354627633626481No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8080.22020128795951346No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7960.2169309718016989No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7550.20575739159583253No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7300.19894423293371888No Hit
ATTCTATGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA7290.1986717065872343No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7080.19294865331105884No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7030.1915860215786361No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7020.19131349523215158No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGACC6840.18640802099542975No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6810.1855904419559761No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6610.18013991502628515No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6550.17850475694737789No Hit
GTACATTCCTGCTGGTGGCTCCAGTTCAGAAACAGTGAGCCCTGCTCAGA6510.1774146515614397No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6500.17714212521495515No Hit
TTCAAGAACAGTGTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA6470.17632454617550153No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6400.17441686175010968No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6330.17250917732471788No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6310.17196412463174876No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6240.17005644020635693No Hit
CTGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC6010.1637883342372124No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTAAGA5860.1597004390399442No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5840.1591553863469751No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5780.1575202282680678No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5590.15234222768486144No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5580.15206970133837688No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5500.14988949056650053No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5480.1493444378735314No Hit
GAAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG5480.1493444378735314No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5420.14770927979462414No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5390.1468917007551705No Hit
GTTCTTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC5380.14661917440868596No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5370.1463466480622014No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTAATCTCTTTTTTGCCTTCTG5370.1463466480622014No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5360.14607412171571688No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5330.14525654267626323No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5320.14498401632977867No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA5300.14443896363680958No Hit
ATTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAATGT5280.1438939109438405No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5240.1428038055579023No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5200.14171370017196414No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5130.1398060157465723No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4960.135173067856335No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4920.13408296247039683No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4880.13299285708445865No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4850.132175278045005No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4790.13054011996609774No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4780.13026759361961318No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4770.12999506727312862No Hit
GCACCGGACCATGCAAGACCTGCACAACTCCTGCTCAAGGAACCTCTATG4750.12945001458015953No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4740.129177488233675No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4690.12781485650125227No Hit
GGAATGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4650.12672475111531406No Hit
ACACAGAATAGCTTGCCTGATTGCCGTATGGTGAGGTGAACAATGTTCCG4540.12372696130398407No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4520.12318190861101497No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4510.12290938226453042No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4480.12209180322507679No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4450.12127422418562314No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4400.1199115924532004No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4360.11882148706726223No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4360.11882148706726223No Hit
GTCATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT4360.11882148706726223No Hit
CCCAATGACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4300.11718632898835495No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4270.11636874994890131No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4270.11636874994890131No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4270.11636874994890131No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT4230.11527864456296313No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4230.11527864456296313No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4230.11527864456296313No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4220.11500611821647858No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4210.11473359186999403No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4190.11418853917702493No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4170.11364348648405585No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4150.11309843379108675No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGAC4150.11309843379108675No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCG4140.1128259074446022No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG4130.11255338109811767No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG4100.11173580205866401No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4100.11173580205866401No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG4030.10982811763327219No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4030.10982811763327219No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4000.10901053859381855No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA3960.10792043320788038No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT3950.10764790686139583No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3950.10764790686139583No Hit
CCCTTAACTTCATGGGATATGTCATTGGGAGTTGGGGCACATTGCCACAG3950.10764790686139583No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3940.10737538051491127No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3910.10655780147545764No Hit
CTGTTTACAGAGAAAGGTCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT3900.1062852751289731No Hit
ATCCAGCATCCAGGGAATTAGTCGTCGGCTATGTCAACGTTAATATGGGC3880.10574022243600399No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG3850.10492264339655036No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3850.10492264339655036No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGACGCGGCGA3820.10410506435709674No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3800.10356001166412764No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC3800.10356001166412764No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACAG3790.10328748531764308No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC3770.102742432624674No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGGTTT3740.10192485358522035No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC3740.10192485358522035No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT3730.1016523272387358No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTCTTGCATGGTCCGGTG3720.10137980089225127No Hit
CTCCCGGGGCCGCTTGGGGCTCTACCGCCCGCTTCTCCGCCTGTTGTACC3690.10056222185279762No Hit
GACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTATGAG3690.10056222185279762No Hit
GGGTAAAGGTTCAGATACTGTTTACAGAGAAAGGTCTTGTAAGTTGGCGA3690.10056222185279762No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3680.10028969550631306No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG3670.10001716915982854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)