FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363418
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18550.5104315141242316No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14410.39651310612022517No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAAAGTCCAA13610.3744998871822529No Hit
CCTCCTGCATATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA11960.32909762312268515No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11220.3087353956050608No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11100.30543341276436503No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10850.2985542818462487No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10840.298279116609524No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC9860.271312923410508No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA9130.2512258611296084No Hit
TCCTCAGGCCATGCAGTGGAACTCCAACACTTTCCACCAAACTCTTCAAG8910.245172225921666No Hit
CTTTTATATAAGATCCCCGCCTCCCAAAGAGTGTGTAAATAATGTCGCGT8810.24242057355441943No Hit
GGATAATATGGTTTGATCCCTTTATCTAAGGGAAAATATTTAGTAACCTT8440.23223945979560726No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8130.22370933745714303No Hit
ATATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7950.21875636319609926No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7630.20995107562091037No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7610.20940074514746104No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7440.20472293612314196No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7400.20362227517624334No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7300.2008706228089968No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG7140.19646797902140237No Hit
CTCTTTGGGAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7130.19619281378467768No Hit
ATATGTTCCTGGGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7030.19344116141743117No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6630.18243455194844505No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCAACACTTT6620.18215938671172038No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6490.1785822386342999No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6260.17225343818963287No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT6250.17197827295290824No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATGTTC6180.17005211629583564No Hit
CCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6180.17005211629583564No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6170.169776951059111No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6110.16812595963876306No Hit
CTGTAAGGGGCCCCACAAATTTCTTACATCTATCAACAATGTCCTCCTGC6080.16730046392858913No Hit
CTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT6050.16647496821841515No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTC5870.1615219939573714No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5850.16097166348392208No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5850.16097166348392208No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5830.1604213330104728No Hit
ACTACATACTCTGGATAATATGGTTTGATCCCTTTATCTAAGGGAAAATA5630.15491802827597972No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5570.1532670368556318No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGGGGATTGGGGACAGAAAGAT5450.14996505401493596No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5420.149139558304762No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5360.14748856688441409No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT5280.14528724499061688No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5280.14528724499061688No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5270.14501207975389221No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5250.1444617492804429No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5230.1439114188069936No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5140.14143493167647173No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACGAT5140.14143493167647173No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5130.14115976643974706No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5110.14060943596629777No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5100.1403342707295731No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTTCCGATGAGTTT5070.13950877501939915No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5060.13923360978267452No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5050.13895844454594986No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG5030.13840811407250053No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5020.1381329488357759No Hit
GGGCTGTACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG4980.13703228788887728No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG4940.13593162694197866No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4910.1351061312318047No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4830.13290480933800747No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCATCAAATGCC4770.13125381791765955No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4760.1309786526809349No Hit
GAATAAAGCCCCGTAAAATTTCCCACCTTATGTGTCCAAGGAATACTAAC4700.12932766126058698No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4660.12822700031368836No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4580.12602567841989115No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4570.1257505131831665No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4560.12547534794644183No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA4540.12492501747299253No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4530.12464985223626787No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA4500.12382435652609391No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4400.1210727041588474No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4350.11969687797522412No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT4280.11777072131815154No Hit
GTATGGATCGGCAGAGGAGACACGAAGGTTCCACGCATGCGCTGATGGCC4260.11722039084470223No Hit
GGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTA4180.11501906895090502No Hit
ACATACTCTGGATAATATGGTTTGATCCCTTTATCTAAGGGAAAATATTT4180.11501906895090502No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4160.11446873847745571No Hit
GTTTAGCCTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT4150.11419357324073105No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4110.11309291229383243No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4080.11226741658365849No Hit
ATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACTGAACA4080.11226741658365849No Hit
GCATATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC4060.11171708611020918No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4050.11144192087348453No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4020.11061642516331056No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGAGGGAGAGGC4000.11006609468986125No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTTACGT3980.10951576421641196No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA3950.10869026850623799No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCCCAG3940.10841510326951335No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3940.10841510326951335No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3930.10813993803278868No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA3920.10786477279606405No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3920.10786477279606405No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3920.10786477279606405No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG3900.10731444232261474No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA3880.10676411184916543No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG3870.10648894661244078No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAAAGAATCGTGGGTCT3870.10648894661244078No Hit
TCCTTGGACACATAAGGTGGGAAATTTTACGGGGCTTTATTCTTCTACGG3870.10648894661244078No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGT3860.10621378137571612No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGTTGG3860.10621378137571612No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3860.10621378137571612No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA3830.10538828566554216No Hit
GTACAACAGGCGGAGAAGCGGGCGGTACAGCCCCAAGCGGCCCCGGGAGG3800.10456278995536819No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA3790.10428762471864354No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT3780.10401245948191888No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3720.10236146806157098No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCCCAGGAACATATTGTACAAAAA3720.10236146806157098No Hit
CCATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACTGAA3720.10236146806157098No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATATTCG3720.10236146806157098No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3700.10181113758812167No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG3670.1009856418779477No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACGATTCT3640.10016014616777374No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATCGTGGGTCTTTTGGGGTTT3640.10016014616777374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)