FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423995
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13580.32028679583485653No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT13040.3075507965895824No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12890.3040130190214507No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT12290.2898619087489239No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10730.25306902204035425No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10240.24151228198445737No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10040.23679524522694845No Hit
CTACGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGG9630.22712531987405513No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9620.22688946803617968No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT9580.2259460606846779No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9280.2188705055484145No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9190.2167478390075355No Hit
GTCAAAGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAG9060.2136817651151547No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9020.2127383577636529No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8950.21108739489852477No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8940.21085154306064932No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT8660.20424769160013678No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA8400.19811554381537516No Hit
CCTCCTTCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8290.19552117359874524No Hit
CTGTTGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCGA8220.19387021073361715No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC8070.19033243316548545No Hit
CTTCCAGGGGACCTGCCTCTTCGTCTAACAACAGTAGTTTCCGGAAGTGT8020.1891531739761082No Hit
CCTTCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC7830.18467198905647472No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7800.1839644335428484No Hit
CCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA7710.18184176700196938No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7530.17759643392021132No Hit
CCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGCCGC7470.17618132289295865No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGCAGCGCC7360.17358695267632873No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7340.17311524900057781No Hit
GTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTT7140.1683982122430689No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7070.16674724937794078No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA6980.16462458283706174No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6940.16368117548555997No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG6920.16320947180980908No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6870.16203021262043182No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT6670.1573131758629229No Hit
ATCATCTTCCTTTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6640.15660562034929656No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATGATTAACTA6570.15495465748416845No Hit
CTCTGTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6510.15353954645691575No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6500.1533036946190403No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6480.15283199094328942No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6370.1502376207266595No Hit
ATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6260.1476432505100296No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6220.1466998431585278No Hit
GCACAGCTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5970.14080354721164165No Hit
ATTTTGTGGTCTATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACA5960.1405676953737662No Hit
ACTGTATGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTA5860.13820917699501173No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5740.13537895494050636No Hit
CCTCCTCCATATCGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5690.13419969575112914No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTTTGGAAGTAATGATTAACTACA5680.1339638439132537No Hit
ATATCGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG5640.13302043656175191No Hit
ATATAAAAGAGAGTCCACACGCAGCGCCTCATTTTGCGGGTCACCATATT5630.13278458472387647No Hit
ACGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5560.13113362185874833No Hit
TCATCTTCCTTTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT5510.1299543626693711No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5500.12971851083149566No Hit
CAAGACTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATC5430.12806754796636755No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGAGCGGCA5410.12759584429061663No Hit
GGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTT5350.12618073326336396No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5290.12476562223611128No Hit
GGATATCCACATTGCGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAAT5260.12405806672248494No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAAAGGA5210.1228788075331077No Hit
ATCAGGGCCCTGAACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAG5110.12052028915435324No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5070.11957688180285145No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5070.11957688180285145No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT5050.11910517812710056No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4970.11721836342409699No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4960.11698251158622154No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4950.1167466597483461No Hit
GGTCTATATGAAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA4930.1162749560725952No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA4920.11603910423471976No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4850.11438814136959163No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4830.11391643769384074No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4820.11368058585596529No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4750.11202962299083716No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4670.1101428082878336No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4670.1101428082878336No Hit
ATCTCATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATC4630.1091994009363318No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4620.10896354909845636No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4600.10849184542270547No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGACAAATCTT4570.10778428990907912No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4430.10448236417882285No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4430.10448236417882285No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4420.10424651234094741No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4420.10424651234094741No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG4410.10401066050307196No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4370.10306725315157017No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4360.10283140131369473No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4320.10188799396219295No Hit
TATCCACATTGCGTGAAAGGAGCGGCAAACCCCAAAAGACCCACAATTCG4300.10141629028644204No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4290.1011804384485666No Hit
TCCACAGAGTGTGTAAATAATGTCGAGTTTGGAAGTAATGATTAACTACA4270.10070873477281571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)